Document Details

Document Type : Thesis 
Document Title :
Molecular Evaluation and Expression Profiling of Her-2/neu in Breast Cancer of Females
تقييم جزيئي وسيماء تعبير للموروثه Her-2/neu في سرطان الثدي عند الإناث
 
Subject : Breast Cancer 
Document Language : English 
Abstract : Almost 25% of breast tumors are due to the amplification of Her-2\neu (human epidermal growth factor receptor -2) gene or the overexpression of its protein product. The oncoprotein Her-2 is a 185 kDa transmembrane receptor that belongs to the epidermal growth factor receptor family. Amplification of the Her-2 oncogene and concomitant overexpression of protein are currently implicated in breast carcinoma as important biomarkers for predicting response to Herceptin (Her-2/neu blocker). It has significant anti-tumor activity both, as a single agent and in combination with chemotherapy in the patient. For this reason, laboratory assessment of Her-2 status is becoming a key step in the optimal management of patients with advanced breast cancer. Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) have emerged as the most two widely used assays to evaluate Her-2 status in breast cancer. In this study, we evaluated IHC and FISH as two clinical assays methods in 50 breast cancer specimens. Overexpression of Her-2/neu protein was found in 29 breast carcinomas and seventeen cases were found to have Her-2/neu gene amplification. High concordance was seen between these two techniques (from 78% to 100%). Some IHC-positive cases did not show Her-2/neu gene amplification by FISH, this was associated with high copy number of chromosome 17 resulting in increased Her-2/neu protein overxepression as detected by QRT-PCR. Quantitative real-time PCR is relatively new and alternative technique for assessing Her-2 gene amplification. A kinetic quantitative polymerase chain reaction method based on fluorescent TaqMan technology has been used in this study to quantify gene amplification in tumor DNA. Among seven samples that have been tested using real-time PCR, extra copies of the gene was seen in five samples. These results correlated well with those of FISH. The use of this technique will make molecular analysis of human cancers simpler and more reliable, and should find broad applications in clinical and research settings. 
Supervisor : Jalaluddin Azam Jalal 
Thesis Type : Master Thesis 
Publishing Year : 1431 AH
2010 AD 
Number Of Pages : 82 
Co-Supervisor : Adeel Gulzar Chaudhary 
Added Date : Wednesday, December 17, 2014 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
منى عبدالقادر الجهنيAl-Juhani, Mona AbdulqadirInvestigatorMaster 

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