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Document Type |
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Thesis |
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Document Title |
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Genetic improvement and Molecular characterization of bacterial isolate producing uricase enzyme التحسین الوراثي والتوصیف الجزیئي لعزلة بكتیریة منتجة لإنزیم الیوریكیز |
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Document Language |
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English |
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Abstract |
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Urate oxidase (EC 1.7.3.3) or uricaseis a tetrameric enzyme of therapeutic
interest implicated in the catalyzed degradation of uric acid, (a final product of
purine catabolism, to allantoin, which is more soluble and more easily to be
excreted than the starting compound). Twenty five isolates of microorganisms
were screened for uricase productionwith medium containing uric acid as the only
carbon source. All isolates were obtained from samples of soil and water,
collected from different places of Jeddah, Saudi Arabia. The degradation of uric
acid in bacterial isolates with different taxonomical properties was investigated.
The amount of uricase was determined by spectrophotometry. Five isolates out of
25 (20%) of the tested isolates produced uricase. The highest uricase producer
was the isolate (SK3) thus; it was selected for more detail studies. Based on its
morphological, biochemical and physiological characteristics, as well as 16srDNA
sequence and phylogenetic analysis, this isolate was identified as Bacillus
cereusSK3. The effects of different factors on the enzyme production were
studied. It was showed that glucose/tryptone broth medium was the most
favorable one, the optimum temperature was at 30°C, and incubation period
required for maximum production was 3 day with aeration level at 150 rpm and
pH 8.0. Different carbon sources and nitrogen sources were used for bacterial
growth and uricase production. Glucose proved to be the best carbon source,
peptone was found to be the best nitrogen source. Moreover, uricase production
was enhanced using mutation. The obtainedmutant grows well and produces
higher uricase, 6times more compared to the original isolate |
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Supervisor |
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Sanaa Tork |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1434 AH
2012 AD |
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Number Of Pages |
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111 |
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Co-Supervisor |
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Magda Mohammad Aly |
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Added Date |
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Monday, December 15, 2014 |
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Researchers
| صفا قطب الفتني | Al-Fattani, Safa Qutub | Investigator | Master | |
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