Document Details

Document Type : Thesis 
Document Title :
HISTOLOGICAL AND ULTRASTRUCTURAL STUDIES ON ABNORMAL SCARS
دراسات نسيجية تركيبية دقيقة على الندب
 
Subject : التئام الجروح 
Document Language : English 
Abstract : Keloid and hypertrophic scars are common complication of wound healing process. In the present study both light and scanning electron microscopy beside immunocytochemistry were used to study the histological characteristic of abnormal scars (hypertrophic scar and keloid). In vitro study was also designed to investigate the morphology of cultured fibroblasts derived from all studied samples. Cell culture showed that fibroblasts of both hypertrophic scar and keloid were found to be much different from those of normal human foreskin. Abnormal scar fibroblasts had abundant spreading cytoplasm, large nuclei and numerous processes with abnormal shaped expanded ends that had facet like ending. Morphometric study showed changes in bipolarity index (L/W) and nuclear cytoplasmic ratio (N/C) of keloid and HSc fibroblasts. Morphology of these cells was studied from Comassi stained sections and scanning microscopy. Histological study showed the characteristic deposition of abnormal thick collagen bundles that differentiate keloid from hypertrophic scars. They showed color variation by Masson Trichrome. In both scars, nodular formation was observed. Newly formed blood vessels were among the characteristic features. They showed endothelial hypertrophy, thickened walls and deposition of homogenous substances and fibrillar collagen in the perivascular tissue. Among the interesting findings, marked cellular infiltration in the perivascular regions and among abnormal collagen was observed. Scanning microscopy of scar tissue also give clear idea about what was observed in paraffin sections. Immunohistochemistry showed the dominance of (CD3) T lymphocytes together with the macrophages (CD68). Numerous mast cells were also observed, in toludine blue stained sections. Mast cells were more numerous in keloid compared to hypertrophic scars. Cellular interaction could be observed as those cells were seen in close relation to each other and to active fibroblasts. This cell–cell interaction or contact may explain what was called in literature (cell talk) via cytokines secreted by these cells or through direct gap junctions. Inducing abnormal scaring in rabbit and mice were tried, but owing to lack of tenacein C failed to occur. In conclusion a question raised which is responsible for increased deposition of collagen in abnormal scars, which of the cells start the activation of fibers blasts, or failure to return to resting state. 
Supervisor : Osama Abdullah Abuzinadah 
Thesis Type : Master Thesis 
Publishing Year : 1430 AH
2009 AD 
Number Of Pages : 303 
Co-Supervisor : Soad Shaker Ali 
Added Date : Monday, May 19, 2014 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ناهد حسن حجرةHajrah, Nahid HassanInvestigatorDoctorate 

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