Document Details

Document Type : Thesis 
Document Title :
Effect of Phytoestrogens Derived from Red Clover (Trifolium pratense L.) in Ovariectomized Rats
تأثير الاستروجينات النباتية المشتقة من البرسيم الأحمر على الفئران مستأصلة المبايض
 
Subject : Rats - Physiology 
Document Language : English 
Abstract : The most common types of osteoporosis is bone loss associated with ovarian hormone deficiency. There is evidence that diets which contain high levels of phytoestrogenic isoflavones such as red clover (Trifolium pratense L.) isoflavones (RCI) are associated with a low incidence of osteoporosis and reduce menopausal symptoms. The objective of this study was to evaluate the preventive effects of RCI on the progression of bone loss induced by estrogen deficiency (ovariectomy) in rats. Sham operation or bilateral ovariectomy (OVX) was performed on female adult rats (n=50). One week after the operation, OVX rats were treated with an oral dose of 20, 40 or 60 mg of RCI daily for 12 weeks. Results showed that the ovariectomy induced significantly increase on of body weight gain percent (BWG%), food intake and fat tissues percent (fat tissues %). Levels of bone specific alkaline phosphatase (B-ALP), N-terminal propeptide (PINP) and osteocalcin significantly elevation, accompanied with significant reduction on estradiol and parathyroid hormone (PTH) levels, as well as bone mineral content (BMC) and bone mineral density (BMD) in OVX group compared with sham group. In addition, OVX induced a rise in the number of osteoclasts and noticeable histological change in the femur sections compared with sham-operated control. Treatment with RCI significantly ameliorated all tested biological bone marker enzymes and hormone assay parameters compared with the OVX untreated rats, as well as improved histological and histochemical alterations induced by OVX. These findings suggest that RCI is effective in reducing bone loss induced by ovariectomy and maintains bone health, probably by reducing bone turnover via inhibition of bone resorption. 
Supervisor : Dr. Hala Abd El-Rahman Hassan Khattab 
Thesis Type : Master Thesis 
Publishing Year : 1434 AH
2013 AD 
Number Of Pages : 130 
Co-Supervisor : Prof. Dr. Mohammed Salleh Ardawi 
Added Date : Wednesday, September 11, 2013 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ريم أحمد عتيقAteeq, Reem AhmadInvestigator  

Files

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 36002.pdf pdf 

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