Document Details

Document Type : Thesis 
Document Title :
POTENTIAL NEUROPROTECTIVE EFFECT OF CARVEDILOL IN EXPERIMENTALLY- INDUCED DIABETIC NEUROPATHY
التأثير الوقائي لعقار الكارفيديلول على الخلايا العصبيه في نموذج تجريبي للإعتلال العصبي السكري
 
Subject : Faculty of Medicine 
Document Language : Arabic 
Abstract : Introduction: Diabetic polyneuropathy (DPN) is very common and affects about 50% of both type1 and type 2 diabetic patients. DPN is the leading cause of diabetes-related hospital admissions and nontraumatic amputations. Oxidative stress may play an important role in the pathophysiology of DPN. Study objective: The current study was carried out to investigate the potential protective effect of carvedilol in experimentally induced diabetic neuropathy on in-vitro & in-vivo models. Material & Methods: Primary dorsal root ganglia (DRG) were cultured from male adult C57BL mice. To induce DPN, the DRG were incubated 24 hours with high glucose (HG) media (45mM). The In-vivo experiment included induction of DPN model by single intraperitoneal (i.p) injection of streptozotocin at dose 50 mg/kg in Sprague Dawley rats. Results: The HG resulted in a decrease in cells viability, a decrease in neuronal length, soma perimeter, soma area and elevation in ATF3 expression which is a marker of the neuronal stress response. Carvedilol (10 µM) significantly protected the DRG from HG-induced changes in DRG morphology and increased the DRG viability compared to HG-treated cells. Carvedilol decreased the activating transcription factor 3 AFT3 expression but non-statistically significant. On the other hand, the in-vivo model resulted in cold allodynia, change in oxidative stress markers such as malondialdehyde, glutathione, and superoxide dismutase. Further more, elevation in nerve growth factor NGF and changes on DRG tissue histology. Carvedilol (1 and 10 mg/kg/d, PO for 45 days) improved the behavioural tests, restored and ameliorated the oxidative stress markers. Conclusion: The current study demonstrates the neuroprotective effect of carvedilol against experimentally induced diabetic neuropathy through an antioxidant mechanism. 
Supervisor : Dr. Rania Mahmoud Magadmi 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2019 AD 
Co-Supervisor : Dr. Ahmed Esmat Ame, 
Added Date : Tuesday, November 19, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
مجاهد عبدالسلام السليمانيAlsulaimani, Mujahid AbdulsalamResearcherMaster 

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