Document Details

Document Type : Thesis 
Document Title :
THE NEUROPROTECTIVE EFFECTS OF MELATONIN ON WHITE MATTER OF MULTIPLE SCLEROSIS INDUCED ANIMAL MODEL
الآثار الوقائية العصبية للميلاتونين للمادة البيضاء في النماذج الحيوانية للتصلب اللويحي
 
Subject : Faculty of Medicine 
Document Language : Arabic 
Abstract : Background: Multiple sclerosis (MS) is a progressive chronic inflammatory autoimmune disease of the myelin sheath, and melatonin is a powerful antioxidant and anti-inflammatory agent that has been shown to be beneficial in MS treatment. The present study was designed to evaluate its protective effect on the demyelination and remyelination process according to gender in a mouse model of MS. Cuprizone was used widely to induce a model of MS, as cuprizone induces artificial demyelination reaction through oligodendrocyte apoptosis and its withdrawal leads to spontaneous remyelination. Methods: Male and female SWR/J mice (N = 78) were obtained from King Fahad Medical Research Center and were divided into three main groups (control, cuprizone and cuprizone + melatonin), which were each further subdivided into males and females. Cuprizone was orally administered in group 2 and 3 at a dose of 400 mg/kg/day by oral gavage for 5 weeks. Moreover, melatonin was intraperitoneally administered in group 3 for 9 weeks at a dose of 80 mg/kg/day. Results: During demyelination stage melatonin exhibited a neuroprotective function through its antagonistic effects on cuprizone-induced neurotoxicity. This was evident through improved locomotor activity, increased antioxidant levels (catalase, superoxide dismutase, glutathione peroxidase, and glutathione), and decreased levels of malondialdehyde and the inflammatory factors interleukin-1beta and tumor necrosis factor-alpha in male and female mice. Moreover, melatonin shown to reserve the oligodendrocytes structure and protect the myelin sheath around the axons. However, the effect of melatonin during the remyelination stage varied between the two genders; male mice were having protective effects by melatonin while no effect was detected in female mice. Conclusion: Melatonin exerted significant and determined effects on pathophysiological process of MS during the de- and remyelination stages in male mice, but these effects were limited in female mice. 
Supervisor : Dr. Badra al-Ghamdi 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2019 AD 
Added Date : Sunday, September 29, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
حنين عبدالباسط أبوطالبAbo Taleb, Hanin AbdulbasetResearcherMaster 

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