Document Details

Document Type : Thesis 
Document Title :
(Antimicrobial Efficacy of The Combined Erbium-Chromium and Diode Laser in Root Canal Therapy (In Vitr
فعالية مضادات الميكروبات الناتجة عن استخدام مركب ليزر الأربيوم كروم وليزر الديود في علاج أقنية جذور الأسنان (دراسة مختبرية)
 
Subject : Faculty of Dentistry 
Document Language : Arabic 
Abstract : Aim: The aim of the present study was to evaluate the antimicrobial efficacy of combined 2780 nm Er,Cr:YSGG and 940 nm diode laser activated irradiation with or without endodontic disinfecting solutions against inoculated E. faecalis biofilm. Materials and Methods: Seventy-five extracted human single rooted teeth with root curvature of ≤ 15° were prepared to size 40, 0.06 taper. Autoclaved specimens were inoculated with Enterococcus faecalis and incubated in a carbon dioxide incubator for 4 weeks except for the negative control (n = 5). Infected teeth were randomly divided into six groups (n = 10): treated with 5.25% sodium hypochlorite for 2 min. (Group 1); 5.25% sodium hypochlorite for 2 min. then 1 min. 2% chlorhexidine (Group 2); dual laser (2780 nm Er,Cr:YSGG and 940 nm diode) with saline for 24 sec. (Group 3); dual laser with 5.25% sodium hypochlorite for 24 sec. (Group 4); dual laser with 5.25% sodium hypochlorite followed by 2% chlorhexidine for 24 sec. (Group 5); saline syringe irrigation for 2 min. (Group 6). Colony forming units were generated from bacterial samples before and after disinfection and evaluated with colony counter device and OpenCFU software. Data were statistically analysed by Kruskal-Wallis and Mann-Whitney U tests for multiple comparison at 0.003 Bonferroni adjusted p value. Results: Complete decontamination of root canals (100%) were achieved by Group 1, Group 2, Group 4 and Group 5 with no statistically significant difference among them (P <0.003). Moreover, dual laser with saline and saline syringe irrigation had inferior antimicrobial effect with reduction rate of 56.25% and 79.39% respectively and no significant difference between them (P > 0.003). Conclusion: The current result indicated that dual laser activated saline had insufficient antimicrobial effect, whereas sufficiently activated sodium hypochlorite with/without chlorohexidine to achieve complete canal decontamination. 
Supervisor : Prof. Sawsan TH. Abu Zeid 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Dr. Khalid A. Merdad 
Added Date : Monday, August 19, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
رهام خالد باعثمانBaothman, Reham KhaledResearcherMaster 

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