Document Details

Document Type : Thesis 
Document Title :
Potentiation of Doxorubicin Cytotoxicity by Calcium Channel Blocker Verapamil in Human Breast Cancer Cells
زيادة فاعلية عقار الدوكسوروبيسين بواسطة الفيراباميل المغلق لقنوات الكالسيوم في خلايا سرطان الثدي البشرية
 
Subject : Faculty of Medicine 
Document Language : Arabic 
Abstract : Breast cancer is a main cause of morbidity and mortality in females in the Arab universe and classified first among Saudi Arabia. Doxorubicin (DOX) is one of the treatment protocol modalities used in treatment of solid tumors such as Hodgkin’s and non-Hodgkin’s lymphoma, sarcomas, breast cancers, and lung cancers. Its medical effectiveness has been, however disadvantaged by harmful effects, including chronic cardiotoxicity and development of DOX resistance. Therefore, this work is directed to potentiate the cytotoxic activity of DOX and to ameliorate the tumor cell resistance by using the calcium channel blocker verapamil (VER). To verify this, have examined DOX cytotoxicity, apoptosis, and cell cycle phase distributing effects of DOX against MCF-7 cell line in presence and absence of the calcium channel blocker verapamil. DOX cellular uptake and P-gp activity has also been examined. Addition of VER showed enhanced cytotoxic effect of DOX against the growth of human breast cancer cell line (MCF-7) cells with IC50 13 µg/ml, compared to 36 µg /ml when DOX was used alone. Moreover, the combination therapy significantly increased the percentage of early apoptosis and cells arrested in G0/G1 phase when compared to DOX alone. In addition, VER significantly increased DOX cellular uptake through inhibition of P-gp activity. In conclusion, VER treatment improved the cytotoxic activity of DOX against the growth of MCF-7 cells and increased its cellular uptake through inhibition of P-gp activity. 
Supervisor : Prof. Abdel-Moneim M. Osman 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Dr. Sameer E. Al-Harthi 
Added Date : Wednesday, July 24, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
مشاعل مسند المطيريAl-Mutairi, Mashael MusnadResearcherMaster 

Files

File NameTypeDescription
 44787.pdf pdf 

Back To Researches Page