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Document Type |
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Thesis |
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Document Title |
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POTENTIATION OF DOXORUBICIN CYTOTOXICITY BY AGED GARLIC EXTRACT IN HUMAN BREAST CANCER CELLS زيادة فعالية عقار الدوكسوروبيسن علي خلايا سرطان الثدي البشرية بواسطة مستخلص الثوم المعمر |
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Subject |
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Faculty of Medicine |
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Document Language |
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Arabic |
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Abstract |
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Breast cancer is one of the most commonly diagnosed type of cancer and different treatments are used to block its advance. However, it still represents a very common cause of death in women. Doxorubicin (DOX) is reported as an effective agent in breast cancer treatment, however it induces many side-effects and development of chemo-resistance which limits its use. For this reason, many laboratories are involved in understanding how it is possible to maximize its cytotoxic effect on cancer cells with decline in its concentrations, considering that one of the possible solutions is to use drug synergy, combining it with natural substances. It has been reported that some dietary components such as: aged garlic extract (AGE), one of the garlic preparations with no strong odor and strict irritating taste, exhibits anticancer effects. Therefore, the aim of current study was to investigate the potential chemosensitizing effect of AGE on DOX in breast cancer cells (MCF-7), with respect to cytotoxicity, cell cycle distribution, apoptosis induction, cellular DOX uptake and p-glycoprotein activity. Adding AGE to MCF-7/DOX cells showed no significant effect at AGE (10 mg/ml). However, co-treatment with AGE (50and 93 mg/ml) showed the antiproliferative effect and enhanced the cytotoxic effect of DOX against the growth of MCF-7 cells with IC50 0.962 and 0.999 µM, compared with 1.85 µM DOX alone, respectively. Moreover, AGE significantly increased percentage of apoptotic cells population that confirmed by Annexin V-FITC &PI technique which found significant increase in late apoptosis. AGE significantly increased DOX cellular uptake and P-gp inhibitions in presence of AGE compared to DOX alone. In conclusion, AGE treatment increased the cytotoxic activity of DOX against the growth of MCF-7 cells. This could be explained by cell cycle distribution, induction of apoptosis, increased DOX cellular uptake as well as P-gp inhibition. |
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Supervisor |
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Dr. Huda AlKreathy |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Co-Supervisor |
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Dr. Fatima Omar Abdulla Kamel |
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Added Date |
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Tuesday, July 16, 2019 |
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Researchers
| نورة فراج الشهري | AlShehri, Noura Farraj | Researcher | Master | |
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