Document Details
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Document Type |
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Thesis |
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Document Title |
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IMPACT OF VITAMIN D DEFICIENCY IN THE PATHOGENESIS OF CARDIOVASCULAR METABOLIC SYNDROME IN RATS تأثير نقص فيتامين د على النشوء المرضي لمتلازمة الأمراض الأيضية القلبية في الجرذان |
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Subject |
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Faculty of Medicine |
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Document Language |
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Arabic |
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Abstract |
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Vitamin D deficiency is a common condition with high prevalence which leads to major health problems. The importance of vitamin D emerges from the different roles and actions of this essential element in the regulation of several body systems and their functions and its deficiency leading to serious problems and diseases. The present study was designed to investigate the effects of vitamin D deficiency on the development of metabolic syndrome. Moreover, the deleterious effect of combined vitamin D deficiency and metabolic syndrome was also studied. The study includes the use of 48 Wister albino rats divided into four groups including; Control group (C), Vitamin D deficient group (VD-), Metabolic syndrome group (MetS) and the Combined vitamin D deficient with metabolic syndrome group (VD- + MetS). The vitamin D deficient animal groups (VD- and VD- + MetS) were fed with vitamin D deficient diet with increased calcium and phosphate concentration in order to attenuate the development of 2ry hyperparathyroidism and determine the direct effects of vitamin D. Metabolic syndrome was induced by adding fructose10% to rats drinking water for 8 weeks. Assessments and laboratory biochemical analysis were performed including; Serum vitamin D level and serum calcium, phosphate & magnesium levels. Blood pressure, serum lipid profile, serum glucose and insulin levels, insulin index and obesity parameters (The percentage of weight gain, body mass index (BMI), abdominal circumference (AC), adiposity index, and total abdominal fat) were also determined. Moreover, serum malondialdehyde (MDA), nitric oxide (NO), creatine kinase MB (CK-MB) and lactate dehydrogenase (LDH) levels were assessed. Whereas all these biochemical testing and assessments were done at zero time and after 8 weeks by the end of the study. Vascular responses on isolated rat thoracic aorta were also investigated. Furthermore, heart weight and histopathological examination were performed. The results obtained revealed that vitamin D deficiency was strongly related to each component of metabolic syndrome. Blood pressure, glucose and insulin levels, triglycerides, total cholesterol, and low-density lipoproteins were significantly increased while high density lipoproteins were significantly decreased. All obesity parameters were significantly increased. Combined vitamin D deficiency and metabolic syndrome induced highly significant increase in CK-MB, LDH, NO, and MDA levels. However, there was no significant difference in CK-MB and NO levels for (VD-) group compared to control. Heart weight was significantly increased as well as the histological examination of the heart showed increased left ventricular thickness. Vascular response to phenylephrine was significantly increased while the vascular response to acetylcholine was significantly decreased. Therefore, the present study clearly demonstrates that vitamin D deficiency is considered as one of the major risky and predisposing factors for cardiovascular metabolic syndrome incidence and could affect the outcome of the disease. Based on the current results, the study recommended further investigations and trials to determinate the underlying molecular pathway vitamin D deficiency effects on Different parameters of metabolic syndrome, endothelial function, and vascular oxidative stress with consideration to factors such as vitamin D receptor polymorphism, gender and age. |
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Supervisor |
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Prof. Mai Abdulalim Abdulsattar |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Co-Supervisor |
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Dr. Fatemah Omar Kamel |
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Added Date |
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Tuesday, April 9, 2019 |
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Researchers
| شيرين خالد محجوب | Mahjob, Shereen Khalid | Researcher | Master | |
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