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Document Type |
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Thesis |
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Document Title |
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Phytochemical and Biological Studies of Citrullus colocynthis دراسات كيميائية وبيولوجية على نبات الحنظل |
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Subject |
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Faculty of Pharmacy |
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Document Language |
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Arabic |
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Abstract |
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Phytochemical study of the EtOAc extract of Citrullus colocynthis fruits (Family Cucurbitaceae) by using different chromatographic techniques led to isolation of four metabolites. Cucurbitacin E (1), 2-O-β-D-glucopyranosylcucurbitacin E (2), 2-O-β-D-glucopyranosylcucurbitacin I (3), and 25-O-acetyl-2-O-β-D-glucopyranosyl cucurbitacin L (4). Their chemical structures were established on the basis of physical, chemical, spectral data 1D (1H and 13C NMR) and 2D NMR (HSQC and HMBC) and comparison with literature data. Cucurbitacin E glucoside (Cu-E glucoside) (2) was found to be the major constituent. Thus, it was evaluated against testosterone-induced prostatic hyperplasia in mice. The results indicate that it decreased prostate weight and prostate index. Histopathological examinations revealed that compound (2) ameliorated pathological changes in prostatic architecture and inhibited the increase in glandular epithelial length induced by testosterone. The observed antiproliferative activity was confirmed by decreased expression of cyclin D1 in treated prostate tissues compared to testosterone-alone group. It showed significant antioxidant activity as evidenced by inhibiting lipid peroxidation accumulation, glutathione depletion and superoxide exhaustion. Further, it exhibited anti-inflammatory activity as it decreased cyclooxygenase-2 and interleukin-1β in prostatic tissues. Masson's trichrome stained of prostate sections showed obvious anti-fibrotic activity which was supported by decreased α-smooth muscle actin protein expression immunohistochemically. In conclusion, Cu-E glucoside inhibits testosterone-induced experimental BPH in mice, at least partly, due to its antiproliferative, antioxidant, anti-inflammatory and antifibrotic effects. |
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Supervisor |
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Prof. Ahmed Abdel Azim Mahdi Abdullatif |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Co-Supervisor |
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Prof. Jamal Abdullah Mohammed Hussein |
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Added Date |
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Friday, February 8, 2019 |
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Researchers
| سلسبيل زامل باشا | Basha, Salsabeel Zamil | Researcher | Master | |
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