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Document Type |
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Thesis |
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Document Title |
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EVALUATION OF SELECTED PRODUCTS CONTAINING NATURAL COMPOUNDS تقييم بعض المنتجات المختارة التى تحتوي على مركبات طبيعية |
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Subject |
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Faculty of Pharmacy |
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Document Language |
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Arabic |
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Abstract |
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An evaluation of fourteen herbal products containing natural compounds and obtained from Saudi Arabian markets has been carried out. Different analytical and pharmacological methods were used. Eight natural products were subjected to evaluation by determination of their total phenolics content using folin-ciocalteuʼs reagent. Additionally, the antimicrobial activity was determined for these products, using agar diffusion method, Gram-nagative, Gram-positive bacteria and Candida albicans fungus. The results of the total phenolics revealed great variation regarding the same product with respect to the solvent used for extraction. The best solvent for extraction of products 2, 3, 6 and product 8 was acetone:water (7:3). Products 1, 4, 5 and product 7 showed the best extraction by using methanol. The highest phenolic contents were detected in product 8 (98.84 mg gallic acid equivalent), and the lowest value was attributed to product 3 (36.95) mg gallic acid equivalent). Other concentrations vary between these two values. Results of the antibacterial activity showed that product 1 showed activity against all the tested organisms except klebsiella pneumonia. Products 5, 6 and 7 showed activity only against Bacillus subtilis; other extracts did not show any activity against the tested organisms under the used experimental conditions.
Three products claimed to contain natural compounds against inflammation were evaluated for their anti-inflammatory activity by using carrageenan induced rat hind paw as an acute model of inflammation to verify the effect of these products . The results showed that product 9 and product 11 showed significant anti-inflammatory results but product 10 had no anti-inflammatory action under the used experimental conditions.
Three products of different batches claiming to contain henna powder were subjected for detection and evaluation of their lawsone content by thin layer chromatography (T.L.C) and gas chromatography-mass spectrometry (GC-MS) different batches of the same product showed different results. Product 13c showed the highest content of lawsone (2458.88 ug/gm), while product 12a showed the lowest content of lawsone (184.9 ug/gm).
Based on our results, it is necessary that herbal products should be subjected to evaluation of their contents and claimed uses, especially if they are non-prescription products, which play an increasingly vital role in Saudis’ health care syste |
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Supervisor |
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Prof. Dr. Nagwa Salah Al-Din Ahmed Al-Shaer |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1439 AH
2018 AD |
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Co-Supervisor |
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Prof. Dr. Hani Zakaria Asfour |
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Added Date |
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Sunday, July 1, 2018 |
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Researchers
| نجوى عبدالحميد معين | Moeen, Najwa AbdulHameed | Researcher | Master | |
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