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Document Type |
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Thesis |
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Document Title |
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PHYTOCHEMICAL INVESTIGATION OF FABA L.SEEDS دراسة فيتوكيميائية لبذور الفول البلدي |
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Subject |
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faculty of Pharmacy |
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Document Language |
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Arabic |
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Abstract |
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Phytochemical study of the methanolic extract of seed coat of Vicia faba L. (Family Fabaceae) using different chromatographic techniques led to the isolation of six known compounds: Ethyl 3-oxobutanoate (1), β-amyrin (2), stigmasterol (3), β-sitosterol glucoside (4), quercetin (5) and rutin (6). Their structures were established on the basis of physical, chemical and spectral data (UV,1D (1H and 13C NMR) and 2D NMR (1H – 1H COSY, HSQC and HMBC) and comparison with literature data. The total MeOH extract (TME) and different fractions were evaluated for their antibacterial and antifungal activities in vitro bioassay. They were evaluated by agar diffusion and microbroth dilution assays using representative standard strains of Gram-positive, Gram-negative bacteria and fungus. The highest activity was observed for TME, followed by n-hexane fraction and then CHCl3 fraction. EtOAc and MeOH Fractions did not show any antimicrobial activity. Comparing the results of n-hexane fraction, CHCl3 fraction and TME and amoxicillin against β-lactam resistant Gram- negative bacterium, E. coli, n-hexane, CHCl3 fractions and TME showed antibacterial activities, with MIC values (5, 5 and 1.25 mg/mL, respectively). n-hexane fraction with MIC value of 5 mg/mL showed interesting antibacterial activities toward β-lactam resistant Gram-negative bacterium, P. aeruginosa, when compared to amoxicillin. As well as, TME showed antifungal activity towards C. albicans with MIC 1.25 mg/mL.The TME of Vicia faba at doses 50 and 100 mg/kg exhibited potent anti-inflammatory activity compared with indomethacin (10 mg/kg). The extracts dose-dependently and significantly decreased the carrageenan-induced increase in paw volume as compared with control rats. The Hepatoprotective activity of TME was assessed. Vicia faba showed hepatoprotective activity against CCl4-hepatotoxicity in rats. It is worth mentioning that this is the first study of anti-inflammatory and hepatoprotective of Vicia faba L. seeds. |
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Supervisor |
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Dr. Nagwa Salah Al-Din Ahmed Al-Shaer |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1439 AH
2018 AD |
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Added Date |
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Tuesday, February 27, 2018 |
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Researchers
| دعاء عمر الاغا | Al-Agha, Doaa Omar | Researcher | Master | |
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