Document Details

Document Type : Thesis 
Document Title :
Crude Extract of Plum(Prunus domestica. L) Inhibits Cell Growth and Induces Apoptosis in Human Colorectal Cancer Cells, HCT116
مستخرج البرقوق الطازج (برقوق شائع) يثبط النمو ويحدث موت ذاتي مبرمج لخلايا سرطان القولون والمستقيم (HCT116)
 
Subject : Faculty of Home Economics 
Document Language : Arabic 
Abstract : Objective: We aimed to investigate the cytotoxic effects of Plum (Prunus domestica. L) on colorectal cancer (CRC) cells (HCT116). Methods: Human colorectal cancer cells (HCT116) and normal oral gingival epithelium cells (MOE-1) were subjected to increasing doses of n-butanol extract of plum (N-BuOH PEX). Cells were then harvested after 24, 48 or 72 h and cell viability was examined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] . Clonogenicity assay was also carried out. Nuclear stains: (Hoechst 33342), Acridine orange/Ethidium bromide double staining, agarose gel electrophoresis and comet assays were performed to assess pro-apoptotic potentiality of the extract. Reactive oxygen species (ROS) production and mitochondrial membrane potential were also measured. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR), using gene-specific primers were performed. Results: Treatment of N-BuOHPEX significantly reduced cell viability in HCT116 cells, promote growth in MOE-1 in a dose- and time-dependent manner (P<0•05). Calculated IC50, after 24, 48 and 72 h, were 1.2, 1 and 0.8 μg/mL respectively. N-BuOH PEX reduced colony formation and exhibited morphologic and biochemical features of apoptotic cell death. The induction of apoptosis was associated with increased ROS production, reduction in mitochondrial membrane potential, induced DNA fragmentation and downregulated expression levels of anti-apoptotic proteins including: (Bcl-2, cyclin D1 and c-Myc) and upregulated expression levels of proapoptotic proteins (Bax and Noxa). Conclusion: The molecular mechanism underlying the antitumor effect of N-BuOH PEX on HCT116 cells could be qualified as one of the promising targets for innovative treatment strategies of CRC. 
Supervisor : Dr. Etab Saleh Alghamdi 
Thesis Type : Master Thesis 
Publishing Year : 1438 AH
2017 AD 
Added Date : Thursday, June 8, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
فـاطمة عبد الرحمن العلكميAlalkami, Fatimah AbdulrahmanResearcherMaster 

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